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| F508del disturbs the dynamics of the nucleotide binding domains of CFTR before and after ATP hydrolysis
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| Abstract The cystic fibrosis transmembrane conductance regulator (CFTR) channel is an ion channel responsible for chloride transport in epithelia and it belongs to the class of ABC transporters. The deletion of phenylalanine 508 (F508del) in CFTR is the most common mutation responsible for cystic fibrosis. Little is known about the effect of the mutation in the isolated nucleotide binding domains (NBDs), on dimer dynamics, ATP hydrolysis and even on nucleotide binding. Using molecular dynamics simulations of the human CFTR NBD dimer, we showed that F508del increases, in the prehydrolysis state, the inter‐motif distance in both ATP binding sites (ABP) when ATP is bound. Additionally, a decrease in the number of catalytically competent conformations was observed in the presence of F508del. We used the subtraction technique to study the first 300 ps after ATP hydrolysis in the catalytic competent site and found that the F508del dimer evidences lower conformational changes than the wild type. Using longer ...
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| Sena moves SC, seeks hearing against Guv's decision of not giving time to submit letter of support
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| The Shiv Sena on Tuesday moved the Supreme Court challenging the Maharashtra governor's decision of not granting it the three days to submit the letter of support for government formation in the state. The lawyers, appearing for the Shiv Sena, told PTI that they have made a request to the registrar for according urgent hearing on Tuesday itself and said they are yet to get a response. The Shiv Sena has termed the governor's Monday decision as unconstitutional, unreasonable and mala fide.
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| Phil Hellmuth loses $20,000 tennis prop bet
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 You know what they say, give a man a fish and he'll eat for a day, but give him a fishing rod and he'll eat until the global warming ultimately renders oceanic lifeforms extinct. Or something like that. In poker terms, however, you only have to give Phil Hellmuth one chance at a bet – seemingly any bet – and he can't resist jumping on the hook. Today, Phil Hellmuth faced off against tennis pro Ellis Guernsey, and stared down the barrel of the young tennis ace's fabled 127mph serve. If Hellmuth could win one point, then he would win $20,000. If not, he'd lose $4,000. Odds of 5/1? You were never going to keep Hellmuth out of that kind of action. Hellmuth actually has form winning prop bets, unlike many poker players. To our minds, the winning ones we can recall stretch to Huck Seed perfecting a gymnastic backflip – his relative was a trained gymnast and brought him up to speed in 24 hours – and, well, Duke Madson's suited walk to Vegas in his recent cinematic triumph. Not only did Hellmuth have form, but he ...
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| Bicknell beats Kornuth to $25k Poker Masters Event #6 title
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 Kristen Bicknell became the first female poker player to win a Poker Masters event as she toppled Chance Kornuth heads-up to triumph in the $25,000-entry NLHE Event in Las Vegas. [Image credit: WPT flickr] Posting one of the biggest titles of her career to date, the partypoker pro took down a final table that at one point looked set up to become a procession for Chance Kornuth's first event win of the Series. Kornuth, who had already finished second in two other Poker Masters events in the past week to take an early lead in the race for the Purple Jacket, was to be denied, however, and in the most spectacular style. With six players making the final table, British high roller Ben Heath would be the first player to be eliminated, running his pocket kings into the pocket aces of Andras Nemeth. Nemeth was back in the game, but with Kornuth using his monster stack to deadly effect, the other players battled to survive rather than mount a serious assault on the chip mountain the 33-year-old player from Denver, ...
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, article language English ( Russian [ translation ] )
